The pipeline runs as follows: 1) digest virtual transcrptome, extract tags, look for internal polyA ./tagger.pl virtual_transcriptome > possible_polyA 2) evaluate empirical tags to look for thise which me be the result of NlaII partial digestion ./partial.pl meta_lib.txt > NlaIII_partial 3) run the alternative splicing analysis ./altsplice.pl metta_lib.txt