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bioinformatics + data visualization

Learning Circos

Bioinformatics and Genome Analysis

Institut Pasteur Tunis Tunisia, December 10 – December 11, 2018

Download course materials
v2.00 6 Dec 2018
Download PDF slides
v2.00 6 Dec 2018

A 2- or 4-day practical mini-course in Circos, command-line parsing and scripting. This material is part of the Bioinformatics and Genome Analysis course held at the Institut Pasteur Tunis.

Updates

I'll be posting corrections to any errors in the material and answers to your questions here, as they come up.

Errata

Answers to Your Questions

SVG output

To create an SVG file (in addition to PNG) use -svg,

> circos -svg

You can view the SVG file with Inkscape. You may want to install the CMU fonts (same as those used in Latex) that come with Circos (see etc/fonts/modern), which are the same fonts used in the PNG files.

Parsing SNPS

I've added an example that shows how to parse sequence alignments and pull out SNPS in Day 1 Lecture 4.

Starting with a file like this

AGACGCGTCGTTGTT
AGACTCGTCGTTGTT

TCAACAATAGCTTTC
TCAACAATAGCTTTC

GACATTCTTACCGCA
GAGATTCTTACCGGA

We get a list of SNPs (where the two records are different) by position.

4 T G
32 G C
43 C G

Tunesian mosaic

I've added section 5 to Day 2 Lecture 4, which shows you how to draw a Tunesian mosaic. You can use this image for your souvenir image, instead of the 9-panel example from section 4.

Download lecture section.

Martin Krzywinski | contact | Canada's Michael Smith Genome Sciences CentreBC Cancer Research CenterBC CancerPHSA
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