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Country flags are pretty colorful and some are even pretty.

Instead of drawing the flag in a traditional way (yawn...), I wanted to draw it purely based on the color proportions in the flag (yay!). There are lots of ways to do this, such as stacked bars, but I decided to go with concentric circles. A few examples are shown below.

Once flags are drawn this way, they can be grouped by similarity in the color proportions.

Check out the posters or read about the method below.

Or, download my country flag color catalog to run your own analysis.

To determine the proportions of colors in each flag, I started with the collection of all country flags in SVG from Wikipedia. The flags are conveniently named using the countries' ISO 3166-2 code. At the time of this project (21 Mar 2017), this repository contained 312 flags, of which I used 256.

I originally wanted to use the flag-icon-css collection, but ran into problems with it. It had flags in only either 1 × 1 or 4 × 3 aspect ratio, which distorted and clipped many flags. Many flags were also inaccurately drawn and had inconsistent use of colors. For example, in Turkey's flag the red inside the white crescent was slightly different than elsewhere in the flag.

I converted the SVG files to high resolution PNG (2,560 pixels in width) and sampled the colors in each flag, keeping only those colors that occupied at least 0.01% of the flag. I apply this cutoff to avoid blends between colors due to anti-aliasing applied in the conversion. When drawing the flags as circles, I only use colors that occupy at least 1% of the flag—this impacts flags that have detailed emblems, such as Belize. I apply some rounding off of the proportions and colors with the same proportion are ordered so that lighter colors (by Lab luminance) are in the center of the circle.

There are various ways to represent the proportions of the flag colors as concentric rings—in other words, to use symbols of different size to encode area.

The accurate way is to have the area of the ring be proportional to the area of the color on the map. The inaccurate way is to encode the area by the the width of the ring. These two cases are the `k=0.5` and `k=1` columns in the figure below, where `k` is the power in `r = a^k` by which the radius of the ring, `r`, is scaled relative to the area, `a`. A perceptual mapping using `k=0.57` has been suggested by some.

My goal here is not to encode the proportions so that they can be read off quantitatively. To find a value of `k`, I drew some flags and looked at their concentric ring representation. For example, with `k=0.57` the Nigerian flag's white center is too large for my eye while for `k=1` it is definitely too small. I liked the proportions for `k=1/\sqrt{2}` but wasn't happy with the fact that flags like France's, which have colors in equal areas, didn't have equal width rings.

In the end I decided on a hybrid approach in which the out radius of color `i` whose area is `a_i` is `r_i = a_i^k + \sum_{j=0}^{i-1} a_j^k` where the colors are sorted so that `a_{i-1} \le a_i`. If I use `k=0.25`, I manage to have flags like France have equal width rings but flags like Nigeria in which the proportions are not equal are closer to the encoding with `k=1/\sqrt{2}`. In this hybrid approach smaller areas, such as the white in the map of Turkey, are exaggerated. Notice that here `k` plays a slightly different role—it's used as the power for each color individually, `\sum a^k`, rather than their sum, `\left({\sum a}\right)^k`.

For the purists this choice of encoding might appear as the crime of the worst sort, representing neither correct (`k=0.5`) nor the conventionally incorrect encoding associated with `k=1`. Think of it this way—I know what rule I'm breaking.

The similarity between two flags is calculated by forming an intersection between the radii positions of the concentric rings of the flags.

For each intersection, the similarity of colors is determined using `\Delta E`, which is the Euclidian distance of the colors in LCH space. I placed less emphasis on luminance and chroma in the similarity calculation by fist transforming the coordinates to `(\sqrt L,\sqrt C, H)`) before calculating color differences. The similarity score is $$ S = \sum \frac{\Delta r}{\sqrt{\Delta E}} $$

Color pairs with `\Delta E < \Delta E_{min} = 5` are considered the same and have an effective `\Delta E = 1`.

I explored different cutoffs and combinations of transforming the color coordinates. This process was informed based on how the order of the flags looked to me.

I decided to start the order with Tonga, since it had the highest average similarity score to all other flags in some of my trials. The flag that is most different from other flags, as measured by the average similarity score, is Israel.

I couldn't find a list of colors in the flags of countries, so I provide my analysis here. Every country's SVG flag was converted into a 2,560 × 1,920 PNG file (4,915,200 pixels). Colors that occupied at least 0.01% of the pixels are listed in their HEX format, followed by the number of pixels they occupy. The fraction of the flag covered by sampled colors is also shown.

DOWNLOAD #code img_pixels sampled_pixels fraction_sampled_pixels hex:pixels,hex:pixels,... ... cm 4366506 4364514 0.999544 FCD116:1513103,007A5E:1456071,CE1126:1395340 cn 4369920 4364756 0.998818 DE2910:4260992,FFDE00:103764 co 4364800 4364800 1.000000 FCD116:2183680,003893:1090560,CE1126:1090560 ...

DOWNLOAD #code1 code2 similarity_score ad ae 0.0108360578506763 ad af 0.0288161214840692 ad ag 0.0510922121861494 ad ai 0.42746294322472 ... zw ye 0.473278765746989 zw yt 0.238101673130705 zw za 0.810589244643825 zw zm 0.573265751850587

We focus on the important distinction between confidence intervals, typically used to express uncertainty of a sampling statistic such as the mean and, prediction and tolerance intervals, used to make statements about the next value to be drawn from the population.

Confidence intervals provide coverage of a single point—the population mean—with the assurance that the probability of non-coverage is some acceptable value (e.g. 0.05). On the other hand, prediction and tolerance intervals both give information about typical values from the population and the percentage of the population expected to be in the interval. For example, a tolerance interval can be configured to tell us what fraction of sampled values (e.g. 95%) will fall into an interval some fraction of the time (e.g. 95%).

Altman, N. & Krzywinski, M. (2018) Points of significance: Predicting with confidence and tolerance *Nature Methods* **15**:843–844.

Krzywinski, M. & Altman, N. (2013) Points of significance: Importance of being uncertain. Nature Methods 10:809–810.

A 4-day introductory course on genome data parsing and visualization using Circos. Prepared for the Bioinformatics and Genome Analysis course in Institut Pasteur Tunis, Tunis, Tunisia.

Data visualization should be informative and, where possible, tasty.

Stefan Reuscher from Bioscience and Biotechnology Center at Nagoya University celebrates a publication with a Circos cake.

The cake shows an overview of a de-novo assembled genome of a wild rice species *Oryza longistaminata*.

The presence of constraints in experiments, such as sample size restrictions, awkward blocking or disallowed treatment combinations may make using classical designs very difficult or impossible.

Optimal design is a powerful, general purpose alternative for high quality, statistically grounded designs under nonstandard conditions.

We discuss two types of optimal designs (D-optimal and I-optimal) and show how it can be applied to a scenario with sample size and blocking constraints.

Smucker, B., Krzywinski, M. & Altman, N. (2018) Points of significance: Optimal experimental design *Nature Methods* **15**:599–600.

Krzywinski, M., Altman, N. (2014) Points of significance: Two factor designs. Nature Methods 11:1187–1188.

Krzywinski, M. & Altman, N. (2014) Points of significance: Analysis of variance (ANOVA) and blocking. Nature Methods 11:699–700.

Krzywinski, M. & Altman, N. (2014) Points of significance: Designing comparative experiments. Nature Methods 11:597–598.

An illustration of the Tree of Life, showing some of the key branches.

The tree is drawn as a DNA double helix, with bases colored to encode ribosomal RNA genes from various organisms on the tree.

All living things on earth descended from a single organism called LUCA (last universal common ancestor) and inherited LUCA’s genetic code for basic biological functions, such as translating DNA and creating proteins. Constant genetic mutations shuffled and altered this inheritance and added new genetic material—a process that created the diversity of life we see today. The “tree of life” organizes all organisms based on the extent of shuffling and alteration between them. The full tree has millions of branches and every living organism has its own place at one of the leaves in the tree. The simplified tree shown here depicts all three kingdoms of life: bacteria, archaebacteria and eukaryota. For some organisms a grey bar shows when they first appeared in the tree in millions of years (Ma). The double helix winding around the tree encodes highly conserved ribosomal RNA genes from various organisms.

Johnson, H.L. (2018) The Whole Earth Cataloguer, Sactown, Jun/Jul, p. 89

An article about keyboard layouts and the history and persistence of QWERTY.

My Carpalx keyboard optimization software is mentioned along with my World's Most Difficult Layout: TNWMLC. True typing hell.

McDonald, T. (2018) Why we can't give up this odd way of typing, BBC, 25 May 2018.